Potential biocontrol efficacy of Trichoderma atroviride with cellulase expression regulator ace1 gene knock-out.

ABSTRACT: The biocontrol function of the repressor of cellulase expression I (ACE1) in Trichoderma atroviride was verified through constructing Δace1 mutant strain by Agrobacterium tumefaciens-mediated transformation. The activities of cell wall-degrading enzymes (cellulase, xylanase, chitinase, β-1,3-glucanase, and protease) in the supernatant of Δace1 mutant strain were distinctly higher than those of control strain, followed with the elevation of related genes transcript levels. Besides, the Δace1 mutant resulted in an elevating transcript level of xyr1, but no obvious change in the expression of cre1, which suggested that ACE1 was negative regulator of the xyr1 transcription, but not involved in cre1 transcription. On core polyketide

SUBMITTER: Fang C 

PROVIDER: S-EPMC6026109 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature