Morphological, genomic and transcriptomic responses of Klebsiella pneumoniae to the last-line antibiotic colistin.

ABSTRACT: Colistin remains one of the few antibiotics effective against multi-drug resistant (MDR) hospital pathogens, such as Klebsiella pneumoniae. Yet resistance to this last-line drug is rapidly increasing. Characterized mechanisms of col^R in K. pneumoniae are largely due to chromosomal mutations in two-component regulators, although a plasmid-mediated col^R mechanism has recently been uncovered. However, the effects of intrinsic colistin resistance are yet to be characterized on a whole-genome level. Here, we used a genomics-based approach to understand the mechanisms of adaptive col^R acquisition in K. pneumoniae. In controlled directed-evolution experiments we observed two distinct paths to colistin resistance acquisition. Whole genome sequencing identified muta

SUBMITTER: Cain AK 

PROVIDER: S-EPMC6026146 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature