Optimization of Immunofluorescent Detection of Bone Marrow Disseminated Tumor Cells.

ABSTRACT:

Background

Cancer metastasis is the primary cause of cancer-related deaths and remains incurable. Current clinical methods for predicting metastatic recurrence are not sensitive enough to detect individual cancer cells in the body; therefore, current efforts are directed toward liquid biopsy-based assays to capture circulating and disseminated tumor cells (CTCs and DTCs) in the blood and bone marrow, respectively. The most promising strategy is fluorescence-based immunostaining using cancer cell-specific markers. However, despite recent efforts to develop robust processing and staining platforms, results from these platforms have been discordant among groups, particularly for DTC detection. While the choice of cancer cell-specific markers is a large factor in this discordance, we h

SUBMITTER: Axelrod HD 

PROVIDER: S-EPMC6026516 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature