The hypoxic tumor microenvironment in vivo selects tumor cells with increased survival against genotoxic stresses.

ABSTRACT: Tumor sensitivity to radiation therapy has been known to be dependent on O₂ concentrations. However, radiosensitivity of naturally occurring hypoxic tumor cells remains to be well fully investigated in direct comparison to that of their adjacent non-hypoxic tumor cells within the same tumor. We developed a hypoxia-sensing xenograft model using the hypoxia-response element (HRE)-driven enhanced green fluorescence protein (EGFP) as a hypoxia reporter to identify hypoxic tumor cells in situ. Here, we have found that naturally hypoxic tumor cells are moderately radioresistant compared to their neighboring non-hypoxic tumor cells in the same tumor. These naturally hypoxic tumor cells are proficient at repairing DNA damages and resist apoptosis induced by genotoxic stresses, which inv

SUBMITTER: Kim H 

PROVIDER: S-EPMC6026555 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature