Development of Versatile Vectors for Heterologous Expression in Bacillus.

ABSTRACT: The discovery of new enzymes for industrial application relies on a robust discovery pipeline. Such a pipeline should facilitate efficient molecular cloning, recombinant expression and functional screening procedures. Previously, we have developed a vector set for heterologous expression in Escherichia coli. Here, we supplement the catalogue with vectors for expression in Bacillus. The vectors are made compatible with a versatile cloning procedure based on type IIS restriction enzymes and T4 DNA ligase, and encompass an effective counter-selection procedure and complement the set of vectors with options for secreted expression. We validate the system with expression of recombinant subtilisins, which are generally challenging to express in a heterologous system. The complement

SUBMITTER: Larsen O 

PROVIDER: S-EPMC6027494 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature