Inhibition of integrin α_Vβ₆ changes fibril thickness of stromal collagen in experimental carcinomas.

ABSTRACT:

Background

Chemotherapeutic efficacy can be improved by targeting the structure and function of the extracellular matrix (ECM) in the carcinomal stroma. This can be accomplished by e.g. inhibiting TGF-β1 and -β3 or treating with Imatinib, which results in scarcer collagen fibril structure in xenografted human KAT-4/HT29 (KAT-4) colon adenocarcinoma.

Methods

The potential role of α_Vβ₆ integrin-mediated activation of latent TGF-β was studied in cultured KAT-4 and Capan-2 human ductal pancreatic carcinoma cells as well as in xenograft carcinoma generated by these cells. The monoclonal α_Vβ₆ integrin-specific monoclonal antibody 3G9 was used to inhibit the α_Vβ₆ integrin activity.

Results

Both KAT-4 and Capan

SUBMITTER: Olof Olsson P 

PROVIDER: S-EPMC6027735 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature