Cryo-EM structure of the serotonin 5-HT_1B receptor coupled to heterotrimeric G_o.

ABSTRACT: G-protein-coupled receptors (GPCRs) form the largest family of receptors encoded by the human genome (around 800 genes). They transduce signals by coupling to a small number of heterotrimeric G proteins (16 genes encoding different α-subunits). Each human cell contains several GPCRs and G proteins. The structural determinants of coupling of G_s to four different GPCRs have been elucidated^1-4, but the molecular details of how the other G-protein classes couple to GPCRs are unknown. Here we present the cryo-electron microscopy structure of the serotonin 5-HT_1B receptor (5-HT_1BR) bound to the agonist donitriptan and coupled to an engineered G_o heterotrimer. In this complex, 5-HT_1BR is in an active state; the intracellular domain

SUBMITTER: Garcia-Nafria J 

PROVIDER: S-EPMC6027989 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature