Development of Arrayed Colonic Organoids for Screening of Secretagogues Associated with Enterotoxins.

ABSTRACT: Enterotoxins increase intestinal fluid secretion through modulation of ion channels as well as activation of the enteric nervous and immune systems. Colonic organoids, also known as colonoids, are functionally and phenotypically similar to in vivo colonic epithelium and have been used to study intestinal ion transport and subsequent water flux in physiology and disease models. In conventional cultures, organoids exist as spheroids embedded within a hydrogel patty of extracellular matrix, and they form at multiple depths, impairing efficient imaging necessary to capture data from statistically relevant sample sizes. To overcome these limitations, an analytical platform with colonic organoids localized to the planar surface of a hydrogel layer was developed. The arrays of densely packed colo

SUBMITTER: Gunasekara DB 

PROVIDER: S-EPMC6028038 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature