A Canonical Biophysical Model of the CsrA Global Regulator Suggests Flexible Regulator-Target Interactions.

ABSTRACT: Bacterial global post-transcriptional regulators execute hundreds of interactions with targets that display varying molecular features while retaining specificity. Herein, we develop, validate, and apply a biophysical, statistical thermodynamic model of canonical target mRNA interactions with the CsrA global post-transcriptional regulator to understand the molecular features that contribute to target regulation. Altogether, we model interactions of CsrA with a pool of 236 mRNA: 107 are experimentally regulated by CsrA and 129 are suspected interaction partners. Guided by current understanding of CsrA-mRNA interactions, we incorporate (i) mRNA nucleotide sequence, (ii) cooperativity of CsrA-mRNA binding, and (iii) minimization of mRNA structural changes to identify an ensemble of likely bin

SUBMITTER: Leistra AN 

PROVIDER: S-EPMC6028588 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature