Ontology highlight
ABSTRACT:
SUBMITTER: Rubio-Cosials A
PROVIDER: S-EPMC6028807 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Rubio-Cosials Anna A Battistini Federica F Gansen Alexander A Cuppari Anna A Bernadó Pau P Orozco Modesto M Langowski Jörg J Tóth Katalin K Solà Maria M
Biophysical journal 20171213 10
Human mitochondrial transcription factor A (TFAM) distorts DNA into a U-turn, as shown by crystallographic studies. The relevance of this U-turn is associated with transcription initiation at the mitochondrial light strand promoter (LSP). However, it has not been yet discerned whether a tight U-turn or an alternative conformation, such as a V-shape, is formed in solution. Here, single-molecule FRET experiments on freely diffusing TFAM/LSP complexes containing different DNA lengths show that a DN ...[more]