Downregulated miR-98-5p promotes PDAC proliferation and metastasis by reversely regulating MAP4K4.

ABSTRACT:

Background

The aberrant expression of microRNAs (miRNAs) has emerged as important hallmarks of cancer. However, the molecular mechanisms underlying the differences of miRNA expression remain unclear. Many studies have reported that miR-98-5p plays vital functions in the development and progression of multiple cancers. However, its role in pancreatic ductal adenocarcinoma (PDAC) remains unknown.

Methods

The expression of miR-98-5p and its specific target gene were determined in human PDAC specimens and cell lines by miRNA qRT-PCR, qRT-PCR and western blot. The effects of miR-98-5p depletion or ectopic expression on PDAC proliferation, migration and invasion were evaluated in vitro using CCK-8 proliferation assays, colony formation assays, wound healing assays and transwell as

SUBMITTER: Fu Y 

PROVIDER: S-EPMC6029016 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature