Competitive suppression of dengue virus replication occurs in chikungunya and dengue co-infected Mexican infants.

ABSTRACT:

Background

Co-circulation of dengue virus (DENV) and chikungunya virus (CHIKV) is increasing worldwide but information on the viral dynamics and immune response to DENV-CHIKV co-infection, particularly in young infants, is scant.

Methods

Blood samples were collected from 24 patients, aged 2 months to 82 years, during a CHIKV outbreak in Mexico. DENV and CHIKV were identified by RT-PCR; ELISA was used to detect IgM and IgG antibodies. CHIKV PCR products were cloned, sequenced and subjected to BLAST analysis. To address serological findings, HMEC-1 and Vero cells were inoculated with DENV-1, DENV-2 and CHIKV alone and in combination (DENV-2-CHIKV and DENV-1-CHIKV); viral titers were measured at 24, 48 and 72 h.

Results

Nine patients (38%) presented co-infection, of who

SUBMITTER: Zaidi MB 

PROVIDER: S-EPMC6029041 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature