Short linear motifs in intrinsically disordered regions modulate HOG signaling capacity.

ABSTRACT:

Background

The effort to characterize intrinsically disordered regions of signaling proteins is rapidly expanding. An important class of disordered interaction modules are ubiquitous and functionally diverse elements known as short linear motifs (SLiMs).

Results

To further examine the role of SLiMs in signal transduction, we used a previously devised bioinformatics method to predict evolutionarily conserved SLiMs within a well-characterized pathway in S. cerevisiae. Using a single cell, reporter-based flow cytometry assay in conjunction with a fluorescent reporter driven by a pathway-specific promoter, we quantitatively assessed pathway output via systematic deletions of individual motifs. We found that, when deleted, 34% (10/29) of predicted SLiMs displayed a significant de

SUBMITTER: Strome B 

PROVIDER: S-EPMC6029073 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature