Expression profiling of Trypanosoma congolense genes during development in the tsetse fly vector Glossina morsitans morsitans.

ABSTRACT:

Background

The tsetse transmitted parasitic flagellate Trypanosoma congolense causes animal African trypanosomosis (AAT) across sub-Saharan Africa. AAT negatively impacts agricultural, economic, nutritional and subsequently, health status of the affected populace. The molecular mechanisms that underlie T. congolense's developmental program within tsetse are largely unknown due to considerable challenges with obtaining sufficient parasite cells to perform molecular studies.

Methods

In this study, we used RNA-seq to profile T. congolense gene expression during development in two distinct tsetse tissues, the cardia and proboscis. Indirect immunofluorescent antibody test (IFA) and confocal laser scanning microscope was used to localize the expression of a putative protein encode

SUBMITTER: Awuoche EO 

PROVIDER: S-EPMC6029126 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature