CPNE1 is a target of miR-335-5p and plays an important role in the pathogenesis of non-small cell lung cancer.

ABSTRACT:

Background

Despite advances in diagnosis and treatment, the survival of non-small cell lung cancer (NSCLC) patients remains poor. There is therefore a strong need to identify potential molecular targets for the treatment of NSCLC. In the present study, we investigated the function of CPNE1 in the regulation of cell growth, migration and invasion.

Methods

Quantitative real-time PCR (qRT-PCR) was used to detect the expression of CPNE1 and miR-335-5p. Western blot and immunohistochemical assays were used to investigate the levels of CPNE1 and other proteins. Flow cytometry was used to determine cell cycle stage and apoptosis. CCK-8 and clonogenic assays were used to investigate cell proliferation. Wound healing, migration and invasion assays were used to investigate the motilit

SUBMITTER: Tang H 

PROVIDER: S-EPMC6029376 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature