Histone demethylase KDM4A and KDM4B expression in granulosa cells from women undergoing in vitro fertilization.

ABSTRACT:

Purpose

To assess expression of the histone demethylases KDM4A and KDM4B in granulosa collected from women undergoing oocyte retrieval and to determine if expression was related to pregnancy outcome.

Methods

Cumulus and mural granulosa cells were obtained from women undergoing oocyte retrieval. KDM4A and KDM4B mRNA expression was determined by qRT-PCR. KDM4A and KDM4B proteins were immunohistochemically localized in ovarian tissue sections obtained from archival specimens.

Results

KDM4A and KDM4B protein was localized to oocytes, granulosa cells, and theca and luteal cells in ovaries from reproductive-aged women. KDM4A and KDM4B mRNA expression was overall higher in cumulus compared to mural granulosa. When comparing granulosa demethylase gene expression, KDM4A and KD

SUBMITTER: Krieg AJ 

PROVIDER: S-EPMC6029998 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature