Lipid rafts are essential for release of phosphatidylserine-exposing extracellular vesicles from platelets.

ABSTRACT: Platelets protect the vascular system during damage or inflammation, but platelet activation can result in pathological thrombosis. Activated platelets release a variety of extracellular vesicles (EVs). EVs shed from the plasma membrane often expose phosphatidylserine (PS). These EVs are pro-thrombotic and increased in number in many cardiovascular and metabolic diseases. The mechanisms by which PS-exposing EVs are shed from activated platelets are not well characterised. Cholesterol-rich lipid rafts provide a platform for coordinating signalling through receptors and Ca²⁺ channels in platelets. We show that cholesterol depletion with methyl-β-cyclodextrin or sequestration with filipin prevented the Ca²⁺-triggered release of PS-exposing EVs. Although calpain activity

SUBMITTER: Wei H 

PROVIDER: S-EPMC6030044 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature