Cold atmospheric plasma causes a calcium influx in melanoma cells triggering CAP-induced senescence.

ABSTRACT: Cold atmospheric plasma (CAP) is a promising approach in anti-cancer therapy, eliminating cancer cells with high selectivity. However, the molecular mechanisms of CAP action are poorly understood. In this study, we investigated CAP effects on calcium homeostasis in melanoma cells. We observed increased cytoplasmic calcium after CAP treatment, which also occurred in the absence of extracellular calcium, indicating the majority of the calcium increase originates from intracellular stores. Application of previously CAP-exposed extracellular solutions also induced cytoplasmic calcium elevations. A substantial fraction of this effect remained when the application was delayed for one hour, indicating the chemical stability of the activating agent(s). Addition of ryanodine and cyclosporin A indic

SUBMITTER: Schneider C 

PROVIDER: S-EPMC6030087 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature