Intradermal injection of low dose human regulatory T cells inhibits skin inflammation in a humanized mouse model.

ABSTRACT: Recent regulatory T cell (Treg) based clinical trials support their therapeutic potential in transplantation and auto-inflammatory diseases. However, large numbers of Treg are needed to accomplish therapeutic efficacy. Local injection at the site of inflammation (targeted delivery) may lower the numbers needed for therapy. We evaluated if local delivery of low numbers of human Treg by intradermal injection was able to prevent skin inflammation, using the humanized mouse huPBL-SCID-huSkin allograft model. A dose of only 1 × 10⁵ freshly isolated, non expanded Treg injected intradermally in close proximity to the transplanted human skin prevented inflammation of the grafted tissue induced by 4 × 10⁷ IP injected human allogeneic PBMCs, (ratio Treg:PBMC = 1:400), as indica

SUBMITTER: Landman S 

PROVIDER: S-EPMC6030170 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature