Covalent Attachment of Enzymes to Paper Fibers for Paper-Based Analytical Devices.

ABSTRACT: Due to its unique material properties, paper offers many practical advantages as a viable platform for sensing devices. In view of paper-based microfluidic biosensing applications, the covalent immobilization of enzymes with preserved functional activity is highly desirable and ultimately challenging. In the present manuscript, we report an efficient approach to achieving the covalent attachment of certain enzymes on paper fibers via a surface-bound network of hydrophilic polymers bearing protein-modifiable sites. This tailor-made macromolecular system consisting of polar, highly swellable copolymers is anchored to the paper exterior upon light-induced crosslinking of engineered benzophenone motifs. On the other hand, this framework contains active esters that can be efficiently modified b

SUBMITTER: Bohm A 

PROVIDER: S-EPMC6030327 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature