Single-Cell Approach to Influenza-Specific CD8⁺ T Cell Receptor Repertoires Across Different Age Groups, Tissues, and Following Influenza Virus Infection.

ABSTRACT: CD8⁺ T cells recognizing antigenic peptides derived from conserved internal viral proteins confer broad protection against distinct influenza viruses. As memory CD8⁺ T cells change throughout the human lifetime and across tissue compartments, we investigated how T cell receptor (TCR) composition and diversity relate to memory CD8⁺ T cells across anatomical sites and immunological phases of human life. We used ex vivo peptide-HLA tetramer magnetic enrichment, single-cell multiplex RT-PCR for both the TCR-alpha (TCRα) and TCR-beta (TCRβ) chains, and new TCRdist and grouping of lymphocyte interactions by paratope hotspots (GLIPH) algorithms to compare TCRs directed against the most prominent human influenza epitope, HLA-A*02:01-M1_58-66 (A2

SUBMITTER: Sant S 

PROVIDER: S-EPMC6030351 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature