Multi-Compartment and Multi-Host Vector Suite for Recombinant Protein Expression and Purification.

ABSTRACT: Recombinant protein expression has become an invaluable tool in basic and applied research. The accumulated knowledge in this field allowed the expression of thousands of protein targets in a soluble, pure, and homogeneous state, essential for biochemical and structural analyses. A lot of progress has been achieved in the last decades, where challenging proteins were expressed in a soluble manner after evaluating different parameters such as host, strain, and fusion partner or promoter strength, among others. In this regard, we have previously developed a vector suite that allows the evaluation of different promoters and solubility enhancer-proteins, through an easy and efficient cloning strategy. Nonetheless, the proper expression of many targets remains elusive, requiring, for example, t

SUBMITTER: Ortega C 

PROVIDER: S-EPMC6030378 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature