Mathematical model of a serine integrase-controlled toggle switch with a single input.

ABSTRACT: Dual-state genetic switches that can change their state in response to input signals can be used in synthetic biology to encode memory and control gene expression. A transcriptional toggle switch (TTS), with two mutually repressing transcription regulators, was previously used for switching between two expression states. In other studies, serine integrases have been used to control DNA inversion switches that can alternate between two different states. Both of these switches use two different inputs to switch ON or OFF. Here, we use mathematical modelling to design a robust one-input binary switch, which combines a TTS with a DNA inversion switch. This combined circuit switches between the two states every time it receives a pulse of a single-input signal. The robustness of the switch is b

SUBMITTER: Pokhilko A 

PROVIDER: S-EPMC6030632 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature