Secreted protein acidic and rich in cysteine-like 1 suppresses metastasis in gastric stromal tumors.

ABSTRACT:

Background

Malignant growth and metastasis of gastrointestinal stromal tumors (GIST) occur in some patients even during the course of treatment, but their mechanisms remains poorly understand at the molecular level so far.

Methods

Profiles of protein expression in gastric GIST tissues were explored using protein microarray analysis, down-regulation of SPARCL1 (secreted protein acidic and rich in cysteine-like protein 1) was validated by RT-qPCR, western blot and immunohistochemistry. The effect of specific shRNA-induced SPARCL1 downregulation on the biological traits of GIST 882 cell was investigated. We then employed a mouse xenograft model to investigate whether the low-expression of SPARCL1 impact the metastasis ability of GIST cells in vivo.

Results

SPARCL1 was si

SUBMITTER: Shen C 

PROVIDER: S-EPMC6030747 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature