Klotho expression is a prerequisite for proper muscle stem cell function and regeneration of skeletal muscle.

ABSTRACT:

Background

Klotho is a well-known anti-aging hormone, which serves as a suppressor of aging through a variety of mechanisms. Aging of skeletal muscle is concomitant with a decrease in muscle stem cell function resulting in impaired regeneration.

Methods

Here we investigate the functional role of the anti-aging hormone Klotho for muscle stem cell function after cardiotoxin-induced injury of skeletal muscle using a klotho hypomorphic mouse line, which is characterized by a premature aging phenotype. Furthermore, we perform floating single myofiber cultures with their adjacent muscle stem cells to investigate the interplay between canonical Wnt signaling and Klotho function.

Results

We demonstrate that muscle stem cell numbers are significantly decreased in klotho hypomo

SUBMITTER: Ahrens HE 

PROVIDER: S-EPMC6030782 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature