A fast fiducial marker tracking model for fully automatic alignment in electron tomography.

ABSTRACT:

Motivation

Automatic alignment, especially fiducial marker-based alignment, has become increasingly important due to the high demand of subtomogram averaging and the rapid development of large-field electron microscopy. Among the alignment steps, fiducial marker tracking is a crucial one that determines the quality of the final alignment. Yet, it is still a challenging problem to track the fiducial markers accurately and effectively in a fully automatic manner.

Results

In this paper, we propose a robust and efficient scheme for fiducial marker tracking. Firstly, we theoretically prove the upper bound of the transformation deviation of aligning the positions of fiducial markers on two micrographs by affine transformation. Secondly, we design an automatic algorithm based on th

SUBMITTER: Han R 

PROVIDER: S-EPMC6030832 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature