Clostridium difficile Whole Genome Sequencing Reveals Limited Transmission Among Symptomatic Children: A Single-Center Analysis.

ABSTRACT:

Background

Although pediatric Clostridium difficile infections (CDIs) are increasing, C. difficile transmission patterns among children are poorly understood.

Methods

We performed whole genome sequencing (WGS) on C. difficile isolates collected from children diagnosed with CDI between December 2012 and December 2013 at a single academic medical center. Genome sequences of isolates from CDIs diagnosed ≥8 weeks after study initiation were compared to all study isolate genome sequences. Among patients with isogenic isolates (≤2-3 core genome single nucleotide variants [SNVs] identified by pairwise SNV analyses), common inpatient and/or outpatient healthcare exposures were investigated.

Results

Among 131 CDIs in 107 children, WGS identified 104 genetically distinct isolat

SUBMITTER: Kociolek LK 

PROVIDER: S-EPMC6030871 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature