HotSpot Wizard 3.0: web server for automated design of mutations and smart libraries based on sequence input information.

ABSTRACT: HotSpot Wizard is a web server used for the automated identification of hotspots in semi-rational protein design to give improved protein stability, catalytic activity, substrate specificity and enantioselectivity. Since there are three orders of magnitude fewer protein structures than sequences in bioinformatic databases, the major limitation to the usability of previous versions was the requirement for the protein structure to be a compulsory input for the calculation. HotSpot Wizard 3.0 now accepts the protein sequence as input data. The protein structure for the query sequence is obtained either from eight repositories of homology models or is modeled using Modeller and I-Tasser. The quality of the models is then evaluated using three quality assessment tools-WHAT_CHECK, PROCHECK and M

SUBMITTER: Sumbalova L 

PROVIDER: S-EPMC6030891 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature