High-quality assembly of the reference genome for scarlet sage, Salvia splendens, an economically important ornamental plant.

ABSTRACT:

Background

Salvia splendens Ker-Gawler, scarlet or tropical sage, is a tender herbaceous perennial widely introduced and seen in public gardens all over the world. With few molecular resources, breeding is still restricted to traditional phenotypic selection, and the genetic mechanisms underlying phenotypic variation remain unknown. Hence, a high-quality reference genome will be very valuable for marker-assisted breeding, genome editing, and molecular genetics.

Findings

We generated 66 Gb and 37 Gb of raw DNA sequences, respectively, from whole-genome sequencing of a largely homozygous scarlet sage inbred line using Pacific Biosciences (PacBio) single-molecule real-time and Illumina HiSeq sequencing platforms. The PacBio de novo assembly yielded a final genome with a scaffol

SUBMITTER: Dong AX 

PROVIDER: S-EPMC6030905 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature