Na/K-ATPase/src complex mediates regulation of CD40 in renal parenchyma.

ABSTRACT:

Background

Recent studies have highlighted a critical role for CD40 in the pathogenesis of renal injury and fibrosis. However, little is currently understood about the regulation of CD40 in this setting.

Methods

We use novel Na/K-ATPase cell lines and inhibitors in order to demonstrate the regulatory function of Na/K-ATPase with regards to CD40 expression and function. We utilize 5/6 partial nephrectomy as well as direct infusion of a Na/K-ATPase ligand to demonstrate this mechanism exists in vivo.

Results

We demonstrate that knockdown of the α1 isoform of Na/K-ATPase causes a reduction in CD40 while rescue of the α1 but not the α2 isoform restores CD40 expression in renal epithelial cells. Second, because the major functional difference between α1 and α2 is the abili

SUBMITTER: Xie JX 

PROVIDER: S-EPMC6031043 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature