Stable isotope informed genome-resolved metagenomics reveals that Saccharibacteria utilize microbially-processed plant-derived carbon.

ABSTRACT:

Background

The transformation of plant photosynthate into soil organic carbon and its recycling to CO₂ by soil microorganisms is one of the central components of the terrestrial carbon cycle. There are currently large knowledge gaps related to which soil-associated microorganisms take up plant carbon in the rhizosphere and the fate of that carbon.

Results

We conducted an experiment in which common wild oats (Avena fatua) were grown in a ¹³CO₂ atmosphere and the rhizosphere and non-rhizosphere soil was sampled for genomic analyses. Density gradient centrifugation of DNA extracted from soil samples enabled distinction of microbes that did and did not incorporate the ¹³C into their DNA. A 1.45-Mbp genome of a Saccharibacteria (TM7) wa

SUBMITTER: Starr EP 

PROVIDER: S-EPMC6031116 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature