HAM-TBS: high-accuracy methylation measurements via targeted bisulfite sequencing.

ABSTRACT:

Background

The ability to accurately and efficiently measure DNA methylation is critical to advance the understanding of this epigenetic mechanism and its contribution to common diseases. Here, we present a highly accurate method to measure methylation using bisulfite sequencing (termed HAM-TBS). This novel method is able to assess DNA methylation in multiple samples with high accuracy in a cost-effective manner. We developed this assay for the FKBP5 locus, an important gene in the regulation of the stress system and previously linked to stress-related disorders, but the method is applicable to any locus of interest.

Results

HAM-TBS enables multiplexed analyses of up to 96 samples and regions spanning 10 kb using the Illumina MiSeq. It incorporates a triplicate bisulfite con

SUBMITTER: Roeh S 

PROVIDER: S-EPMC6031184 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature