Repurposing High-Throughput Image Assays Enables Biological Activity Prediction for Drug Discovery.

ABSTRACT: In both academia and the pharmaceutical industry, large-scale assays for drug discovery are expensive and often impractical, particularly for the increasingly important physiologically relevant model systems that require primary cells, organoids, whole organisms, or expensive or rare reagents. We hypothesized that data from a single high-throughput imaging assay can be repurposed to predict the biological activity of compounds in other assays, even those targeting alternate pathways or biological processes. Indeed, quantitative information extracted from a three-channel microscopy-based screen for glucocorticoid receptor translocation was able to predict assay-specific biological activity in two ongoing drug discovery projects. In these projects, repurposing increased hit rates by 50- to 2

SUBMITTER: Simm J 

PROVIDER: S-EPMC6031326 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature