Determining mechanical features of modulated epithelial monolayers using subnuclear particle tracking.

ABSTRACT: Force generation within cells, mediated by motor proteins along cytoskeletal networks, maintains the function of multicellular structures during homeostasis and when generating collective forces. Here, we describe the use of chromatin dynamics to detect cellular force propagation [a technique termed SINK (sensors from intranuclear kinetics)] and investigate the force response of cells to disruption of the monolayer and changes in substrate stiffness. We find that chromatin dynamics change in a substrate stiffness-dependent manner within epithelial monolayers. We also investigate point defects within monolayers to map the impact on the strain field of a heterogeneous monolayer. We find that cell monolayers behave as a colloidal assembly rather than as a continuum since the data fit an expon

SUBMITTER: Armiger TJ 

PROVIDER: S-EPMC6031382 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature