Exploiting sorghum genetic diversity for enhanced aluminum tolerance: Allele mining based on the Alt_SB locus.

ABSTRACT: Root damage due to aluminum (Al) toxicity restricts crop production on acidic soils, which are extensive in the tropics. The sorghum root Al-activated citrate transporter, SbMATE, underlies the Al tolerance locus, Alt_SB, and increases grain yield under Al toxicity. Here, Alt_SB loci associated with Al tolerance were converted into Amplification Refractory Mutation System (ARMS) markers, which are cost effective and easy to use. A DNA pooling strategy allowed us to identify accessions harboring rare favorable Alt_SB alleles in three germplasm sets while greatly reducing genotyping needs. Population structure analysis revealed that favorable Alt_SB alleles are predominantly found in subpopulations enriched with guinea sorghums, supporting a possible W

SUBMITTER: Hufnagel B 

PROVIDER: S-EPMC6031643 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature