STIM1 Knockout Enhances PDGF-Mediated Ca²⁺ Signaling through Upregulation of the PDGFR⁻PLCγ⁻STIM2 Cascade.

ABSTRACT: Platelet-derived growth factor (PDGF) has mitogenic and chemotactic effects on fibroblasts. An increase in intracellular Ca²⁺ is one of the first events that occurs following the stimulation of PDGF receptors (PDGFRs). PDGF activates Ca²⁺ elevation by activating the phospholipase C gamma (PLCγ)-signaling pathway, resulting in ER Ca²⁺ release. Store-operated Ca²⁺ entry (SOCE) is the major form of extracellular Ca²⁺ influx following depletion of ER Ca²⁺ stores and stromal interaction molecule 1 (STIM1) is a key molecule in the regulation of SOCE. In this study, wild-type and STIM1 knockout mouse embryonic fibroblasts (MEF) cells were used to investigate the role of STIM1 in PDGF-induced Ca²⁺ oscillation and its func

SUBMITTER: Huang TY 

PROVIDER: S-EPMC6032054 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature