Understanding the Virulence of Staphylococcus pseudintermedius: A Major Role of Pore-Forming Toxins.

ABSTRACT: Staphylococcus pseudintermedius is responsible for severe and necrotizing infections in humans and dogs. Contrary to S. aureus, the pathophysiological mechanisms involved in this virulence are incompletely understood. We previously showed the intracellular cytotoxicity induced after internalization of S. pseudintermedius. Herein, we aimed to identify the virulence factors responsible for this cytotoxic activity. After addition of filtered S. pseudintermedius supernatants in culture cell media, MG63 cells, used as representative of non-professional phagocytic cells (NPPc), released a high level of LDH, indicating that the cytotoxicity was mainly mediated by secreted factors. Accordingly, we focused our attention on S. pseudintermedius toxins. In silico<

SUBMITTER: Maali Y 

PROVIDER: S-EPMC6032551 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature