Position-specific ¹³ C/¹² C analysis of amino acid carboxyl groups - automated flow-injection analysis based on reaction with ninhydrin.

ABSTRACT:

Rationale

The fundamental level of stable isotopic knowledge lies at specific atomic positions within molecules but existing methods of analysis require lengthy off-line preparation to reveal this information. An automated position-specific isotope analysis (PSIA) method is presented to determine the stable carbon isotopic compositions of the carboxyl groups of amino acids (δ¹³ C_CARBOXYL values). This automation makes PSIA measurements easier and routine.

Methods

An existing high-performance liquid chromatography (HPLC) gas handling interface/stable isotope ratio mass spectrometry system was modified by the addition of a post-column derivatisation unit between the HPLC system and the interface. The post-column reaction was optimised to yield CO₂

SUBMITTER: Fry B 

PROVIDER: S-EPMC6032851 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature