Purification of influenza virus-like particles using sulfated cellulose membrane adsorbers.

ABSTRACT:

Background

Vaccines based on virus-like particles (VLPs) are an alternative to inactivated viral vaccines that combine good safety profiles with strong immunogenicity. In order to be economically competitive, efficient manufacturing is required, in particular downstream processing, which often accounts for major production costs. This study describes the optimization and establishment of a chromatography capturing technique using sulfated cellulose membrane adsorbers (SCMA) for purification of influenza VLPs.

Results

Using a design of experiments approach, the critical factors for SCMA performance were described and optimized. For optimal conditions (membrane ligand density: 15.4 µmol cm^-2, salt concentration of the loading buffer: 24 mmol L^-1 NaCl, and

SUBMITTER: B Carvalho S 

PROVIDER: S-EPMC6033026 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature