Secretagogin protects Pdx1 from proteasomal degradation to control a transcriptional program required for β cell specification.

ABSTRACT:

Objective

Specification of endocrine cell lineages in the developing pancreas relies on extrinsic signals from non-pancreatic tissues, which initiate a cell-autonomous sequence of transcription factor activation and repression switches. The steps in this pathway share reliance on activity-dependent Ca²⁺ signals. However, the mechanisms by which phasic Ca²⁺ surges become converted into a dynamic, cell-state-specific and physiologically meaningful code made up by transcription factors constellations remain essentially unknown.

Methods

We used high-resolution histochemistry to explore the coincident expression of secretagogin and transcription factors driving β cell differentiation. Secretagogin promoter activity was tested in response to genetically manip

SUBMITTER: Malenczyk K 

PROVIDER: S-EPMC6034064 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature