Three-dimensional volume fluorescence-imaging of vascular plasticity in adipose tissues.

ABSTRACT:

Objective

The vascular system is central to sustaining tissue survival and homeostasis. Blood vessels are densely present in adipose tissues and exert essential roles in their metabolism. However, conventional immunohistochemistry methods have intrinsic limitations in examining the 3D vascular network in adipose tissues as well as other organs in general.

Methods

We established a 3D volume fluorescence-imaging technique to visualize the vasculatures in mouse adipose tissues by combining the optimized steps of whole-mount immunolabeling, tissue optical clearing, and lightsheet volume fluorescence-imaging. To demonstrate the strength of this novel imaging procedure, we comprehensively assessed the intra-adipose vasculatures under obese conditions or in response to a cold chall

SUBMITTER: Cao Y 

PROVIDER: S-EPMC6034070 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature