N-Gemini peptides: cytosolic protease resistance via N-terminal dimerization of unstructured peptides.

ABSTRACT: Herein we describe a synthetically simple strategy for increasing the lifetime of unstructured peptides in cytosolic environment via dimerization at the N-terminus to block threading into the catalytic cleft of cytosolic proteases. We establish this approach with kinase substrates, allowing for phosphorylation in cells as a demonstration of protease resistance.

SUBMITTER: Fayer EL 

PROVIDER: S-EPMC6034174 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature