Assessing Plasmodium falciparum transmission in mosquito-feeding assays using quantitative PCR.

ABSTRACT:

Background

Evaluating the efficacy of transmission-blocking interventions relies on mosquito-feeding assays, with transmission typically assessed by microscopic identification of oocysts in mosquito midguts; however, microscopy has limited throughput, sensitivity and specificity. Where low prevalence and intensity mosquito infections occur, as observed during controlled human malaria infection studies or natural transmission, a reliable method for detection and quantification of low-level midgut infection is required. Here, a semi-automated, Taqman quantitative PCR (qPCR) assay sufficiently sensitive to detect a single-oocyst midgut infection is described.

Results

Extraction of genomic DNA from Anopheles stephensi midguts using a semi-automated extraction process was shown t

SUBMITTER: Wang CYT 

PROVIDER: S-EPMC6034226 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature