Siegesbeckia pubescens Makino inhibits Pam₃CSK₄-induced inflammation in RAW 264.7 macrophages through suppressing TLR1/TLR2-mediated NF-κB activation.

ABSTRACT:

Background

Siegesbeckia pubescens Makino (SP) is one of the important plant origins for the anti-inflammatory Chinese herbal medicine of Siegesbeckiae Herba. The current investigations indicated that the anti-inflammatory effects of SP were associated with the toll-like receptors (TLRs)-mediated nuclear factor-κB (NF-κB) and the mitogen-activated protein kinase (MAPK) signaling pathways.

Methods

Raw 264.7 macrophages were pretreated with the 50% ethanol extract of SP (SPE, 50-200 µg/mL) and then co-treated with Pam₃CSK₄ (200 ng/mL) for another 12 h. The inhibitory effect of SPE on Pam₃CSK₄-stimulated NO release and post-inflammatory cytokines secretions were determined using Griess reagent and Elisa kits, respectively. The i

SUBMITTER: Sang W 

PROVIDER: S-EPMC6034227 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature