DDIT4 promotes gastric cancer proliferation and tumorigenesis through the p53 and MAPK pathways.

ABSTRACT:

Background

Gastric cancer (GC) is one of the most common malignancies worldwide, particularly in China. DNA damage-inducible transcript 4 (DDIT4) is a mammalian target of rapamycin inhibitor and is induced by various cellular stresses; however, its critical role in GC remains poorly understood. The present study aimed to investigate the potential relationship and the underlying mechanism between DDIT4 and GC development.

Methods

We used western blotting, real-time polymerase chain reaction, and immunohistochemical or immunofluorescence to determine DDIT4 expression in GC cells and tissues. High-content screening, cell counting kit-8 assays, colony formation, and in vivo tumorigenesis assays were performed to evaluate cell proliferation. Flow cytometry was used to investigate

SUBMITTER: Du F 

PROVIDER: S-EPMC6034313 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature