Non-linear hierarchy of the quorum sensing signalling pathway in bloodstream form African trypanosomes.

ABSTRACT: Trypanosoma brucei, the agents of African trypanosomiasis, undergo density-dependent differentiation in the mammalian bloodstream to prepare for transmission by tsetse flies. This involves the generation of cell-cycle arrested, quiescent, stumpy forms from proliferative slender forms. The signalling pathway responsible for the quorum sensing response has been catalogued using a genome-wide selective screen, providing a compendium of signalling protein kinases phosphatases, RNA binding proteins and hypothetical proteins. However, the ordering of these components is unknown. To piece together these components to provide a description of how stumpy formation arises we have used an extragenic suppression approach. This exploited a combinatorial gene knockout and overexpression strategy to asse

SUBMITTER: McDonald L 

PROVIDER: S-EPMC6034907 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature