A comprehensive analysis of the Korean fir (Abies koreana) genes expressed under heat stress using transcriptome analysis.

ABSTRACT: Korean fir (Abies koreana), a rare species endemic to South Korea, is sensitive to climate change. Here, we used next-generation massively parallel sequencing technology and de novo transcriptome assembly to gain a comprehensive overview of the Korean fir transcriptome under heat stress. Sequencing control and heat-treated samples of Korean fir, we obtained more than 194,872,650 clean reads from each sample. After de novo assembly and quantitative assessment, 42,056 unigenes were generated with an average length of 908 bp. In total, 6,401 differentially expressed genes were detected, of which 2,958 were up-regulated and 3,443 down-regulated, between the heat-treated and control samples. A gene ontology analysis of these unigenes revealed heat-stress-related terms, such as "response to stim

SUBMITTER: Hwang JE 

PROVIDER: S-EPMC6035224 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature