Retinal Configuration of ppR Intermediates Revealed by Photoirradiation Solid-State NMR and DFT.

ABSTRACT: Pharanois phoborhodopsin (ppR) from Natronomonas pharaonis is a transmembrane photoreceptor protein involved in negative phototaxis. Structural changes in ppR triggered by photoisomerization of the retinal chromophore are transmitted to its cognate transducer protein (pHtrII) through a cyclic photoreaction pathway involving several photointermediates. This pathway is called the photocycle. It is important to understand the detailed configurational changes of retinal during the photocycle. We previously observed one of the photointermediates (M-intermediates) by in situ photoirradiation solid-state NMR experiments. In this study, we further observed the ¹³C cross-polarization magic-angle-spinning NMR signals of late photointermediates such as O- and N'-intermediates by illuminati

SUBMITTER: Makino Y 

PROVIDER: S-EPMC6035295 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature