Control of triple-negative breast cancer using ex vivo self-enriched, costimulated NKG2D CAR T cells.

ABSTRACT:

Background

Triple-negative breast cancer (TNBC) is an aggressive disease that currently lacks effective targeted therapy. NKG2D ligands (NKG2DLs) are expressed on various tumor types and immunosuppressive cells within tumor microenvironments, providing suitable targets for cancer therapy.

Methods

We applied a chimeric antigen receptor (CAR) approach for the targeting of NKG2DLs expressed on human TNBCs. Lentiviral vectors were used to express the extracellular domain of human NKG2D that binds various NKG2DLs, fused to signaling domains derived from T cell receptor CD3 zeta alone or with CD27 or 4-1BB (CD137) costimulatory domain.

Results

Interleukin-2 (IL-2) promoted the expansion and self-enrichment of NKG2D-redirected CAR T cells in vitro. High CD25 expression on fi

SUBMITTER: Han Y 

PROVIDER: S-EPMC6035420 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature