Rational modification of tricarboxylic acid cycle for improving L-lysine production in Corynebacterium glutamicum.

ABSTRACT:

Background

Oxaloacetate (OAA) and L-glutamate are essential precursors for the biosynthesis of L-lysine. Reasonable control of all potentially rate-limiting steps, including the precursors supply rate, is of vital importance to maximize the efficiency of L-lysine fermentation process.

Results

In this paper, we have rationally engineered the tricarboxylic acid (TCA) cycle that increased the carbon yield (from 36.18 to 59.65%), final titer (from 14.47 ± 0.41 to 23.86 ± 2.16 g L^-1) and productivity (from 0.30 to 0.50 g L^-1 h^-1) of L-lysine by Corynebacterium glutamicum in shake-flask fermentation because of improving the OAA and L-glutamate availability. To do this, the phosphoenolpyruvate-pyruvate-oxaloacetate (PEP-pyruvate-OAA) node's genes pp

SUBMITTER: Xu JZ 

PROVIDER: S-EPMC6035423 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature